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ap sema3a plasmid  (Addgene inc)


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    Addgene inc ap sema3a plasmid
    Ap Sema3a Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ap sema3a plasmid/product/Addgene inc
    Average 91 stars, based on 3 article reviews
    ap sema3a plasmid - by Bioz Stars, 2026-02
    91/100 stars

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    Figure 2: Successful construction of lentiviruses overexpressing <t>Sema3A-HIF1a</t> fusion protein. The CDS areas of SEMA3A and HIF1A genes were constructed into LV-EGFP shuttle plasmid to generate oeLenti-Sema3A and oeLenti-HIF1a viruses, separately. The two CDS areas were also linked together to generate oeLenti-Sema3A-HIF1a viruses. iPSC-MSCs were infected with the three types of lentiviruses, and 3 days later, cells were harvested for Western blotting using HIF1a antibody (A), Sema3A antibody (B), or both (C, D). CDS: Coding sequence; HIF1a: Hypoxia inducible factor 1 subunit alpha; iPSCs: Induced pluripotent stem cells; LV-EGFP: Lentivirus-enhanced green fluorescent protein; MSCs: Mesenchymal stem cells; nc: Negative control; oeLenti: Overexpression lentiviruses; Sema3A: Semaphorin 3A; WB: Western blotting.
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    Figure 2: Successful construction of lentiviruses overexpressing <t>Sema3A-HIF1a</t> fusion protein. The CDS areas of SEMA3A and HIF1A genes were constructed into LV-EGFP shuttle plasmid to generate oeLenti-Sema3A and oeLenti-HIF1a viruses, separately. The two CDS areas were also linked together to generate oeLenti-Sema3A-HIF1a viruses. iPSC-MSCs were infected with the three types of lentiviruses, and 3 days later, cells were harvested for Western blotting using HIF1a antibody (A), Sema3A antibody (B), or both (C, D). CDS: Coding sequence; HIF1a: Hypoxia inducible factor 1 subunit alpha; iPSCs: Induced pluripotent stem cells; LV-EGFP: Lentivirus-enhanced green fluorescent protein; MSCs: Mesenchymal stem cells; nc: Negative control; oeLenti: Overexpression lentiviruses; Sema3A: Semaphorin 3A; WB: Western blotting.
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    Figure 2: Successful construction of lentiviruses overexpressing Sema3A-HIF1a fusion protein. The CDS areas of SEMA3A and HIF1A genes were constructed into LV-EGFP shuttle plasmid to generate oeLenti-Sema3A and oeLenti-HIF1a viruses, separately. The two CDS areas were also linked together to generate oeLenti-Sema3A-HIF1a viruses. iPSC-MSCs were infected with the three types of lentiviruses, and 3 days later, cells were harvested for Western blotting using HIF1a antibody (A), Sema3A antibody (B), or both (C, D). CDS: Coding sequence; HIF1a: Hypoxia inducible factor 1 subunit alpha; iPSCs: Induced pluripotent stem cells; LV-EGFP: Lentivirus-enhanced green fluorescent protein; MSCs: Mesenchymal stem cells; nc: Negative control; oeLenti: Overexpression lentiviruses; Sema3A: Semaphorin 3A; WB: Western blotting.

    Journal: Chinese Medical Journal

    Article Title: Semaphorin 3A-hypoxia inducible factor 1 subunit alpha co-overexpression enhances the osteogenic differentiation of induced pluripotent stem cells-derived mesenchymal stem cells in vitro

    doi: 10.1097/cm9.0000000000000612

    Figure Lengend Snippet: Figure 2: Successful construction of lentiviruses overexpressing Sema3A-HIF1a fusion protein. The CDS areas of SEMA3A and HIF1A genes were constructed into LV-EGFP shuttle plasmid to generate oeLenti-Sema3A and oeLenti-HIF1a viruses, separately. The two CDS areas were also linked together to generate oeLenti-Sema3A-HIF1a viruses. iPSC-MSCs were infected with the three types of lentiviruses, and 3 days later, cells were harvested for Western blotting using HIF1a antibody (A), Sema3A antibody (B), or both (C, D). CDS: Coding sequence; HIF1a: Hypoxia inducible factor 1 subunit alpha; iPSCs: Induced pluripotent stem cells; LV-EGFP: Lentivirus-enhanced green fluorescent protein; MSCs: Mesenchymal stem cells; nc: Negative control; oeLenti: Overexpression lentiviruses; Sema3A: Semaphorin 3A; WB: Western blotting.

    Article Snippet: Overexpression of Sema3A and HIF1a fusion protein in iPSC-MSCs The coding sequence (CDS) areas of SEMA3A andHIF1A genes were linked with a three (GGGGS; G, glycine; S, serine) linker and inserted into lentivirus-enhanced green fluorescent protein (LV-EGFP) shuttle plasmid (Addgene).

    Techniques: Construct, Plasmid Preparation, Infection, Western Blot, Sequencing, Negative Control, Over Expression

    Figure 3: Sema3A and HIF1a co-expression enhanced the survival of iPSC-MSCs, and augmented their osteogenic differentiation. (A) The iPSC-MSCs infected with oeLenti-Sema3A, oeLenti-HIF1a, and oeLenti-Sema3A-HIF1a were subjected to MTT assay. iPSC-MSCs cultured in osteogenic induction media for 7 or 14 days were subjected for determining ALP activity (B) and mineralization via alizarin-red staining (C). ∗P < 0.05. ALP: Alkaline phosphatase; HIF1a: Hypoxia inducible factor 1 subunit alpha; iPSCs: Induced pluripotent stem cells; MSCs: Mesenchymal stem cells; MTT: Methyl thiazoleterazolium; nc: Negative control; ns: Not significant; oeLenti: Overexpression lentiviruses; Sema3A: Semaphorin 3A.

    Journal: Chinese Medical Journal

    Article Title: Semaphorin 3A-hypoxia inducible factor 1 subunit alpha co-overexpression enhances the osteogenic differentiation of induced pluripotent stem cells-derived mesenchymal stem cells in vitro

    doi: 10.1097/cm9.0000000000000612

    Figure Lengend Snippet: Figure 3: Sema3A and HIF1a co-expression enhanced the survival of iPSC-MSCs, and augmented their osteogenic differentiation. (A) The iPSC-MSCs infected with oeLenti-Sema3A, oeLenti-HIF1a, and oeLenti-Sema3A-HIF1a were subjected to MTT assay. iPSC-MSCs cultured in osteogenic induction media for 7 or 14 days were subjected for determining ALP activity (B) and mineralization via alizarin-red staining (C). ∗P < 0.05. ALP: Alkaline phosphatase; HIF1a: Hypoxia inducible factor 1 subunit alpha; iPSCs: Induced pluripotent stem cells; MSCs: Mesenchymal stem cells; MTT: Methyl thiazoleterazolium; nc: Negative control; ns: Not significant; oeLenti: Overexpression lentiviruses; Sema3A: Semaphorin 3A.

    Article Snippet: Overexpression of Sema3A and HIF1a fusion protein in iPSC-MSCs The coding sequence (CDS) areas of SEMA3A andHIF1A genes were linked with a three (GGGGS; G, glycine; S, serine) linker and inserted into lentivirus-enhanced green fluorescent protein (LV-EGFP) shuttle plasmid (Addgene).

    Techniques: Expressing, Infection, MTT Assay, Cell Culture, Activity Assay, Staining, Negative Control, Over Expression

    Figure 4: Sema3A and HIF1a co-expression upregulated the expression of osteogenic markers in iPSC-MSCs. The iPSC-MSCs were infected with oeLenti-Sema3A, oeLenti-HIF1a, and oeLenti-Sema3A-HIF1a, and then switched into osteogenic induction media. Cells were harvested 2 or 4 days following the induction, and their mRNAs and proteins were extracted for analyzing the mRNA (A, B) and protein levels of Opn and Ocn (C, D). ∗P < 0.05. HIF1a: Hypoxia inducible factor 1 subunit alpha; iPSCs: Induced pluripotent stem cells; MSCs: Mesenchymal stem cells; nc: Negative control; ns: Not significant; Ocn: Osteocalcin; oeLenti: Overexpression lentiviruses; Opn: Osteopontin; Sema3A: Semaphorin 3A.

    Journal: Chinese Medical Journal

    Article Title: Semaphorin 3A-hypoxia inducible factor 1 subunit alpha co-overexpression enhances the osteogenic differentiation of induced pluripotent stem cells-derived mesenchymal stem cells in vitro

    doi: 10.1097/cm9.0000000000000612

    Figure Lengend Snippet: Figure 4: Sema3A and HIF1a co-expression upregulated the expression of osteogenic markers in iPSC-MSCs. The iPSC-MSCs were infected with oeLenti-Sema3A, oeLenti-HIF1a, and oeLenti-Sema3A-HIF1a, and then switched into osteogenic induction media. Cells were harvested 2 or 4 days following the induction, and their mRNAs and proteins were extracted for analyzing the mRNA (A, B) and protein levels of Opn and Ocn (C, D). ∗P < 0.05. HIF1a: Hypoxia inducible factor 1 subunit alpha; iPSCs: Induced pluripotent stem cells; MSCs: Mesenchymal stem cells; nc: Negative control; ns: Not significant; Ocn: Osteocalcin; oeLenti: Overexpression lentiviruses; Opn: Osteopontin; Sema3A: Semaphorin 3A.

    Article Snippet: Overexpression of Sema3A and HIF1a fusion protein in iPSC-MSCs The coding sequence (CDS) areas of SEMA3A andHIF1A genes were linked with a three (GGGGS; G, glycine; S, serine) linker and inserted into lentivirus-enhanced green fluorescent protein (LV-EGFP) shuttle plasmid (Addgene).

    Techniques: Expressing, Infection, Negative Control, Over Expression

    Figure 5: iPSC-MSCs modified by Sema3A-HIF1a overexpression grew better and preferred to osteogenic differentiation on HA scaffolds. (A) Two days post the seed of iPSC-MSCs onto HA scaffolds under a SEM (Bar = 20 mm). The iPSC-MSCs were cultured with or without (Blank) HA scaffold for 14 days, and then harvested for qRT-PCR (B, C), Western blotting (D, E), ALP activity (F), and MTT assays (G). ∗P < 0.05. ALP: Alkaline phosphatase; HA: Hydroxyapatite; HIF1a: Hypoxia inducible factor 1 subunit alpha; iPSCs: Induced pluripotent stem cells; MSCs: Mesenchymal stem cells; MTT: Methyl thiazoleterazolium; qRT-PCR: Quantitative real-time polymerase chain reaction; Ocn: Osteocalcin; oeLenti: Overexpression lentiviruses; Opn: Osteopontin; Sema3A: Semaphorin 3A; SEM: Scanning electron microscope.

    Journal: Chinese Medical Journal

    Article Title: Semaphorin 3A-hypoxia inducible factor 1 subunit alpha co-overexpression enhances the osteogenic differentiation of induced pluripotent stem cells-derived mesenchymal stem cells in vitro

    doi: 10.1097/cm9.0000000000000612

    Figure Lengend Snippet: Figure 5: iPSC-MSCs modified by Sema3A-HIF1a overexpression grew better and preferred to osteogenic differentiation on HA scaffolds. (A) Two days post the seed of iPSC-MSCs onto HA scaffolds under a SEM (Bar = 20 mm). The iPSC-MSCs were cultured with or without (Blank) HA scaffold for 14 days, and then harvested for qRT-PCR (B, C), Western blotting (D, E), ALP activity (F), and MTT assays (G). ∗P < 0.05. ALP: Alkaline phosphatase; HA: Hydroxyapatite; HIF1a: Hypoxia inducible factor 1 subunit alpha; iPSCs: Induced pluripotent stem cells; MSCs: Mesenchymal stem cells; MTT: Methyl thiazoleterazolium; qRT-PCR: Quantitative real-time polymerase chain reaction; Ocn: Osteocalcin; oeLenti: Overexpression lentiviruses; Opn: Osteopontin; Sema3A: Semaphorin 3A; SEM: Scanning electron microscope.

    Article Snippet: Overexpression of Sema3A and HIF1a fusion protein in iPSC-MSCs The coding sequence (CDS) areas of SEMA3A andHIF1A genes were linked with a three (GGGGS; G, glycine; S, serine) linker and inserted into lentivirus-enhanced green fluorescent protein (LV-EGFP) shuttle plasmid (Addgene).

    Techniques: Over Expression, Cell Culture, Quantitative RT-PCR, Western Blot, Activity Assay, Real-time Polymerase Chain Reaction, Microscopy